yetJ [2019-04-22 17:27:36]

pH-dependent calcium leak

locus: BSU07200

pI: 9.14

mw: 23.68 kDa

protein length: 214 aa

gene length: 645 bp

function: export of calcium, homeostasis

product: pH-dependent calcium leak

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.4|Metal ion homeostasis/ Other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 787,992 → 788,636

The protein

Protein family

• BI1 family (single member, according to UniProt)

Structure

• [PDB|4PGU] [Pubmed|24904158]
• [PDB|6NQ7] (lipidic cubic phase) [Pubmed|30930064]
• [PDB|6NQ8] (D171E mutant) [Pubmed|30930064]
• [PDB|6NQ9] (D195E mutant) [Pubmed|30930064]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|F0A217DB65ED7A7539F775FEBC5D88718B4958BD|yetJ]-[gene|9B8857B4493AD36A2395372B00273244D291BBA7|yetK]
  description: [Pubmed|22383849]

• genes: [gene|F0A217DB65ED7A7539F775FEBC5D88718B4958BD|yetJ]
  description: [Pubmed|22383849]

• genes: [gene|1F2E7B30C04E8C58DDC3583A9C912BD480E979F7|yezD]-[gene|F0A217DB65ED7A7539F775FEBC5D88718B4958BD|yetJ]-[gene|9B8857B4493AD36A2395372B00273244D291BBA7|yetK]
  description: [Pubmed|22383849]

• genes: [gene|1F2E7B30C04E8C58DDC3583A9C912BD480E979F7|yezD]-[gene|F0A217DB65ED7A7539F775FEBC5D88718B4958BD|yetJ]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16885442], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

Biological materials

Mutant

• MGNA-B461 (yetJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1460 NBRP B. subtilis, Japan]
• BKE07200 (Δ[gene|F0A217DB65ED7A7539F775FEBC5D88718B4958BD|yetJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAAAAATCCTCCTAA, downstream forward: _UP4_GGCATTTTGAGCAGTGATGA
• BKK07200 (Δ[gene|F0A217DB65ED7A7539F775FEBC5D88718B4958BD|yetJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAAAAATCCTCCTAA, downstream forward: _UP4_GGCATTTTGAGCAGTGATGA

References

• 24904158,22383849,30930064,16885442